ISOLATION, IDENTIFICATION AND ANTIBIOGRAM STUDY OF BACTERIA ASSOCIATED WITH QUAIL FECES IN SYLHET SADAR, BANGLADESH

Abstract

The current research was conducted for isolation, identification of isolates, and antibiogram study of quail feces isolated from different quail farms and hatchery in Sylhet, Bangladesh. The data used for this study were randomly collected from three different farms and hatchery in the Sylhet district. A total of 90 samples (30 samples from each farm) were settled for bacterial isolation. In this study, A series of culture and biochemical tests were carried out for the isolation and identification of isolated isolates. The antibiotic sensitivity tests of E. coli, Salmonella spp., and Pseudomonas spp. were determined using the Kirby Bauer discdiffusion method. Out of 90 samples, E. coli was found 74 (82.22%), and the highest number of 26(86.67%) was observed in shibganj Bazar Sylhet. The second most pathogen of Salmonella spp. was recorded in Hashem quail and poultry breeders with the prevalence of 23(76.67%), whereas the lowest number of Pseudomonas spp. was obtained in sarkar quail and poultry hatchery 16.67%, respectively. The antibiotic susceptibility test exposed that E. coli were 100% sensitive to Colistin sulfate and Neomycin, whereas cloxacillin showed 100% resistance. Salmonella spp. and Pseudomonas spp. were shown 100% resistant to Amoxicillin, Ampicillin, and cloxacillin. We isolates the three most pathogenic isolates from different farm and hatchery, and these bacteria create a severe public health risk.